Centrifuge

Floor standing high-speed freezing centrifuge (item number: 10AS)

Application oriented, high-speed stability control, intelligent and easy-to-use

1、 Product Overview and Positioning

The 10AS floor standing high-speed freezing centrifuge is designed for multidisciplinary scenarios such as molecular and cell biology, clinical testing, biotechnology, and biological product preparation. It focuses on four major capabilities: large sample size, high efficiency, strong temperature control, and full protection. Its maximum speed is 10000 r/min and maximum relative centrifugal force is 15730 × g. It can handle various processes such as blood component separation, cell sedimentation/collection, protein precipitation, nucleic acid purification, and fermentation broth clarification, meeting the high-frequency and high reliability requirements of scientific research and pilot applications. The equipment is based on a floor standing low center of gravity structure and a perfect dynamic balance design, which maintains low noise, low vibration, and low temperature rise during long-term high-speed operation, reducing operational intensity and maintenance burden, and improving sample processing throughput per unit time. This model adopts a stainless steel centrifugal chamber, an all steel body, and a multi-layer explosion-proof structure, supplemented by multiple self inspection and protection logics such as overspeed/overtemperature/imbalance/door cover, designed to meet the compliance requirements of clinical and GxP scenarios.

2、 Core Separation and Temperature Control Principle

10AS establishes a stable and repeatable centrifugal field through high-speed rotation, enabling effective stratification of particles with different densities and sizes in the suspension system according to sedimentation differences; Combined with a temperature control system with a wide range of -20 ℃ to+40 ℃ and a precision of ± 1 ℃, it achieves low-temperature protection for thermosensitive samples such as proteins, peptides, virus particles, etc. The dual cycle refrigeration process and fluorine free compressor unit (R404A) ensure that the chamber temperature remains low under high-speed operating conditions. The document states that the chamber temperature can still be maintained at ≤ -5 ℃ at the highest speed, and it has two strategies: pre cooling and rapid cooling. The typical performance is a cooling rate of about 5 minutes from 25 ℃ to 4 ℃, significantly reducing preparation time and improving cycle efficiency.

3、 Structure and Security Architecture

The whole machine adopts a fully steel structure and a 304 stainless steel cavity, combined with multi-layer explosion-proof design, to enhance mechanical strength and corrosion resistance; The door cover is driven by automatic induction adsorption and independent servo motor, with emergency unlocking plan for power outage, taking into account both high-frequency opening and closing and human-machine safety in case of abnormalities; The built-in logic of overspeed, overtemperature, imbalance, and door cover interlock in the system, combined with multi-point real-time monitoring, forms a complete machine safety loop from "prevention detection response".

4、 Drive system and dynamic balance

The 10AS is directly driven by a brushless variable frequency motor, which has the advantages of high torque, fast response, anti-interference, low noise, and maintenance free, and can meet the dual requirements of "fast speed up/down and long-term stability"; The spring cone sleeve connection method achieves 360 ° locking between the rotary head and the spindle, with no directional loading and unloading. The rotor replacement is fast, stable, and reliable, reducing the occasional risks and time costs caused by repeated assembly.

5、 Thermal Management and Energy Efficiency

R404A environmentally friendly refrigerant, dual cycle heat exchange route, and optimized internal airflow organization constitute a systematic thermal management capability of "forced cooling+low thermal resistance+fast recovery". For frequent opening or continuous batch operations, the system uses pre cooling and constant temperature maintenance modes to reduce thermal shock, improve temperature regression speed, and ensure inter batch consistency and activity fidelity.

6、 Human computer interaction and programmatic control

The 10AS is equipped with a 5-inch high-definition LCD touch screen, which displays core parameters such as rotor number, speed, time, temperature, upshift gear, and downshift gear on the same screen, making it easy to quickly check and track during method development and routine operation; And provide programmatic management for 10 sets of custom up/down speed modes or 100 sets of up/down speed curve combinations to meet the centrifugal dynamics requirements of different sample characteristics (such as using "soft brake curve" for easy resuspension precipitation, using "slow rise curve" for high viscosity systems, etc.), significantly improving repeatability.

7、 Key technical parameters (according to document caliber)

Maximum speed: 10000 r/min

Maximum relative centrifugal force (RCF): 15730 × g

Maximum capacity: 6 × 1000 mL

Timing range: 1 min to 9 h and 59 min

Speed accuracy: ± 20 r/min

Temperature control range: -20 ℃ to+40 ℃

Temperature control accuracy: ± 1 ℃

Noise: ≤ 65 dB (A)

Power supply: AC 380 V, 50 Hz, 20 A

Dimensions: Approximately 800 × 740 × 930 mm

Net weight: approximately 310 kg

The above data is used for scheme configuration, site and power condition verification.

8、 Rotor and accessory ecology (example list)

To meet the high g-value requirements ranging from large volume processing to medium volume processing, 10AS can be paired with multiple types of angular rotors and horizontal rotors (some models and upper performance limits are as follows). The actual selection is subject to the task scenario and methodology:

NO 05165 angular rotor: 8000 r/min, 6 × 1000 mL, maximum centrifugal force 14550 × g;

NO 05166 angular rotor: 8500 r/min, 6 × 1000 mL (carbon fiber), maximum centrifugal force 15810 × g;

NO 05161 angular rotor: 8000 r/min, 6 × 500 mL, maximum centrifugal force 11740 × g;

NO 05159 angular rotor: 10000 r/min, 6 × 300 mL, maximum centrifugal force 15730 × g;

NO 05205 horizontal rotor+NO 05605/05606 test cup: 4200 r/min, 6 × 1000 mL (round cup/oval cup), maximum centrifugal force 5100 × g.

Explanation: Different combinations of rotors and test cups have strict limits on the maximum allowable speed and maximum RCF; During assembly, follow the three-level verification principle of "rotor identification rated limit sample balancing", and implement maintenance and scrap cycle management according to the instructions.

9、 Typical application process and methodological points

1) Separation of blood and body fluids:

Using a horizontal rotor paired with a 1000 mL round/elliptical cup, clear stratification is often achieved through a medium low speed medium time strategy; The plan for platelet retention should reduce braking intensity to avoid resuspension.

2) Cell collection and supernatant clarification:

For eukaryotic/prokaryotic cell sedimentation, the angle rotor 6 × 1000 mL or 6 × 500 mL protocol is common; For high-density cultures, it is recommended to first clear the solution at a low speed for a short period of time, and then proceed to the final step at a high speed to reduce particle aggregation.

3) Protein precipitation/nucleic acid extraction:

For temperature sensitive systems, it is recommended to use pre cooling and constant temperature maintenance, and the rise/fall speed curve should be "slow rise+soft brake" to reduce shear; A small volume high g-value solution can be used with a 6 × 300 mL angular rotor (10000 r/min).

10、 Operating Procedure (SOP Example)

Before starting the machine: check the rotor and limit value, inspect the cavity and shaft, and balance the sample (weight difference ≤ 0.1 g);

Parameter settings: speed or RCF, time, temperature, acceleration/deceleration curve, and door cover lock status;

In operation: monitoring screen display and alarm; Abnormal (unbalanced/over temperature), stop the machine according to the prompts and recheck the loading;

After completion: Wait until the rotor is completely stationary before opening the cover; Take the supernatant/precipitate according to the methodology;

Record and trace: Save/export programs and running parameters for easy replication of experiments and quality audits.

11、 Installation conditions and compliance certification

Power supply three-phase AC 380 V/50 Hz/20 A, it is recommended to configure an independent circuit and voltage stabilization/grounding; Equipment occupation and transportation channels shall be reserved at a safe distance of 800 × 740 × 930 mm. The document shows that the model has passed ISO9001, ISO13485, EN61010, EN61326 and other systems/standards, and has corresponding filing and production qualifications (applicable to relevant model groups), providing a basic guarantee for quality management and electrical safety in medical and pharmaceutical scenarios.

12、 Maintenance and lifespan management

Daily: Clean the chamber and rotor, promptly wipe off any residual solution;

Rotor: Establish a usage ledger and determine whether to stop or scrap according to the rated life and appearance (cracks/corrosion/deformation);

Refrigeration: Regularly inspect the compressor and condensing system to maintain clean and unobstructed heat exchange channels;

Electrical: Inspect the power terminals and grounding to avoid voltage fluctuations and poor contact;

Shutdown: Long term use of semi open door cover and dry dehumidification to prevent mold and condensation.

13、 Investment return and comparative advantage

One machine with multiple capabilities: covering the mainstream task spectrum of clinical research pilot scale under the combination of 10000 r/min/15730 × g and 6 × 1000 mL capacity;

Low noise steady state: ≤ 65 dB (A) and floor standing low center of gravity structure, suitable for long-term high-speed operation;

Fast pace: Pre cooling+rapid cooling (25 ℃ → 4 ℃ for about 5 minutes) reduces preparation time and improves shift turnover;

Programmatic: Reuse 10/100 sets of acceleration and deceleration strategies to reduce manpower for secondary methodological development;

Full protection: self inspection+interlocking+emergency unlocking, reducing human-machine risks, and strengthening compliance and auditability.

Manufacturer: Changsha Shi Ge Yan Biotechnology Co., Ltd; Warranty for 3 years, only replacement without repair.